京西稻的百年传承与发展

京西稻是清康熙、雍正、乾隆等亲自选育的优良品种,在与紫禁城并重的“三山五园”政治中心内外种植,具有多重价值属性,属于皇家御稻。从2000年起,基于北京水资源匮乏等原因,京西稻种植面积大幅度降至134 hm²左右,处于濒危状态。在保护传承过程中,京西稻入选中国重要农业文化遗产目录。在新修订的《北京历史文化名城保护条例》中,又将京西稻列入遗产保护目录。在海淀区,京西稻已经深入公园、学校、社区、酒店等地,在教育文化中发挥着农业以外的作用。     

Bulk pollen pollination in maize for efficient construction of introgression populations with high genome coverage

Introgression populations consist of a set of introgression lines or families, constructed by continuous backcrossing to the recurrent parent, while carrying a limited number of chromosome segments from a donor parent in their genomes. Increasing the genome coverage is an important aim when constructing introgression population. In this study, we proposed bulk pollen pollination (BPP) method and used it to increase the genome coverage of a maize introgression population. The results showed that the genome coverage of the introgression population constructed using BPP method reached 100% at BC₃ generation, which accorded with the simulation result. The BPP‐based BC₃F_1:2 population could identify most quantitative trait loci (QTL) detected using the F_2:3 population, especially major QTL. Simulation analysis showed that the genome coverage of introgression population increased with the increase of population size and the number of bulked plants, and decreased with the increase of backcross generation. Our results proved the reliability of the BPP‐based introgression population in increasing genome coverage and detecting QTL, and provided references for constructing high‐coverage introgression populations.     

株行距及穴苗数的配置对寒地水稻产量和品质的影响

为明确垦稻26和龙粳31高产优质栽培的株行距和穴苗数处理,采取裂区设计进行试验研究。结果表明,最优群体因品种而异,垦稻26在株距10cm、行距30cm、5苗/穴产量最佳,龙粳31则以株距13.3cm、行距27cm、9苗/穴产量最佳;垦稻26在5苗/穴、行株距30cm×10cm处理,有利于提高稻米的碾磨品质,不利于改善稻米的外观品质,营养品质较低,利于改善稻米的食味品质,且食味评分值高达86.5,显著高于最低处理;龙粳31在5苗/穴、行株距27cm×13.3cm处理,碾磨品质较高,不利于营养品质和外观品质的改善,食味品质得到明显改善,食味评分值高达86.0,与最低处理差异达显著水平。因此,高产优质栽培要因品种选择适宜的穴苗数和株行距,是实现水稻高产优质最为快捷、最为经济有效的措施。     

香蕉与B基因组相关的SCAR标记研究

香蕉(Musa L.)是由2个二倍体野生种Musa acuminata Colla(AA基因型)和Musa balbisiana Colla(BB基因型)种内或种间杂交进化而来,其B基因组中带有重要的优良基因。利用与香蕉B基因组相关的gypsy-IRAP分子标记,成功开发了一对SCAR引物,适用于鉴定尖叶蕉(AAw)、长梗蕉(BB)、香牙蕉(AAA)、贡蕉(AAcv)、大蕉、粉蕉(ABB)、粉大蕉(ABB)、龙牙蕉(AAB)以及四倍体香蕉(AAAB)等是否含有B基因组。     

Pigeonpea improvement: An amalgam of breeding and genomic research

In the past five decades, constant research has been directed towards yield improvement in pigeonpea resulting in the deployment of several commercially acceptable cultivars in India. Though, the genesis of hybrid technology, the biggest breakthrough, enigma of stagnant productivity still remains unsolved. To sort this productivity disparity, genomic research along with conventional breeding was successfully initiated at ICRISAT. It endowed ample genomic resource providing insight in the pigeonpea genome combating production constraints in a precise and speedy manner. The availability of the draft genome sequence with a large‐scale marker resource, oriented the research towards trait mapping for flowering time, determinacy, fertility restoration, yield attributing traits and photo‐insensitivity. Defined core and mini‐core collection, still eased the pigeonpea breeding being accessible for existing genetic diversity and developing stress resistance. Modern genomic tools like next‐generation sequencing, genome‐wide selection helping in the appraisal of selection efficiency is leading towards next‐generation breeding, an awaited milestone in pigeonpea genetic enhancement. This paper emphasizes the ongoing genetic improvement in pigeonpea with an amalgam of conventional breeding as well as genomic research.     

大花君子兰叶绿体基因组及其特征

采用Illumina MiSeq测序平台对大花君子兰（Clivia miniata）叶片总DNA进行测序，通过组装获得了其叶绿体基因组（cpDNA）全长序列（158 114 bp）。对其cpDNA注释得到135个基因，包含87个蛋白编码基因、40个tRNA基因和8个rRNA基因。采用生物信息学方法对获得的cpDNA进行简单序列重复（SSR）分析和密码子偏好性分析。结果显示：①大花君子兰cpDNA中共有61个SSR位点，其中单核苷酸、二核苷酸、三核苷酸、四核苷酸、五核苷酸和六核苷酸重复数分别为38、9、2、8、3和1个，多数SSR分布在基因间隔区；②大花君子兰cpDNA密码子偏爱以A或U（T）结尾，亮氨酸使用频率最高，半胱氨酸使用频率最低。基于24种植物的cpDNA全长和23种植物的叶绿体ycf2基因序列进行系统发育分析，结果显示大花君子兰与石蒜科植物在同一分支，显示最近的亲缘关系，支持大花君子兰属于石蒜科。基于叶绿体ycf2的系统发育分析结果与基于cpDNA全长的系统发育分析研究结果大部分相同，支持ycf2基因可以代替cpDNA全长用于植物系统发育分析。     

Revisiting rice breeding methods – evaluating the use of rapid generation advance (RGA) for routine rice breeding

Rice production needs to increase in the future in order to meet increasing demands. The development of new improved and higher yielding varieties more quickly will be needed to meet this demand. However, most rice breeding programmes in the world have not changed in several decades. In this article, we revisit the evidence in favour of using rapid generation advance (RGA) as a routine breeding method. We describe preliminary activities at the International Rice Research Institute (IRRI) to re-establish RGA on a large scale as the main breeding method for irrigated rice breeding. We also describe experiences from the early adoption at the Bangladesh Rice Research Institute. Evaluation of RGA breeding lines at IRRI for yield, flowering time and plant height indicated transgressive segregation for all traits. Some RGA lines were also higher yielding than the check varieties. The cost advantages of using RGA compared to the pedigree method were also empirically determined by performing an economic analysis. This indicated that RGA is several times more cost effective and advantages will be realized after 1 year even if facilities need to be built. Based on our experience, and previous independent research empirically testing the RGA method in rice, we recommend that this method should be implemented for routine rice breeding in order to improve breeding efficiency.     

Preparation and characterization of gluten-free sheeted doughs and noodles with zein and rice flour containing different amylose contents

This study aimed to explore the feasibility of zein as a gluten alternative in preparing sheeted doughs with rice flours containing different amylose contents (12, 19, and 26%) that were successfully slit into noodle strands. Rice-zein mixtures with lower amylose contents exhibited lower pasting parameters and the high amylose paste samples possessed more elastic properties. Higher water absorption was observed in the low amylose rice-zein mixture whereas the use of zein were effective in increasing the stability of rice dough regardless of amylose content. In case of thermal conductivity, the noodle doughs prepared with high amylose rice-zein mixture had low thermal conductivity, probably implying a long cooking time. The structure of the rice-zein noodles had a tendency to become firmer with increasing levels of amylose by showing greater breaking stress and resistance to extension that could be correlated to reduced cooking loss.     

水稻条纹病毒在玉米和水稻上流行差异分析

为了明确水稻条纹病毒(RSV)在水稻和玉米上发生程度差异明显的原因,从一个侧面了解RSV流行本质,2004年在洪泽进行田间试验。采用黄盘诱集、盘扑、盘刮、肉眼计数等方法比较武育粳3号和掖单13上灰飞虱侵入和消长动态,结果表明两者均只有一个成虫侵入高峰,单位面积迁入虫量前者是后者的3.6倍,单株平均虫量相近。Dot-ELISA法测定灰飞虱带毒率为40%,成虫迁移扩散高峰期22d内两者接毒量约为每天百株137头带毒虫。武育粳3号有二代若虫发生,掖单13则无。逐日调查发病进程,结果显示水稻发病率为60%,玉米为0。室内抗性鉴定,掖单13对RSV的抗性比武育粳3号高4个级别。综合以上结果,寄主品种抗性决定了RSV在水稻和玉米上流行状况,二代若虫重复侵染是RSV在水稻上重发的另一主要原因。     

多效疏导素对水稻秧苗素质及产量影响的研究

研究了多效疏导素水溶液对水稻秧苗素质和产量的影响。结果表明,采用适宜的浓度穴10‰雪多效疏导素水溶液对水稻秧苗素质有显著提高,可提高水稻产量达8.0%;过高的浓度,则会明显抑制水稻秧苗生长,造成减产。